Lymphocyte membrane IgG and secreted IgG are structurally and allotypically distinct.

Lymphocyte membrane IgG and secreted IgG are structurally and allotypically distinct

We have demonstrated that there are structurally distinct membrane and secreted IgG2a immunoglobulin molecules. The membrane heavy chain is both larger and more acidic than the secreted molecule. This difference is not a result of different N-glycosidic-linked oligosaccharide chains. The membrane heavy chain also is antigenically different from its secreted homologue. This is based on the fact ...

Congenital TOXOPLASMOSIS (IgG-IgM) TOXOPLASMA IgG TOXOCARA IgG LEISHMANIA IgG ECHINOCOCCUS IgG CYSTICERCOSIS IgG TRICHINELLA IgG SCHISTOSOMA IgG FASCIOLA IgG HUMAN DIAGNOSTIC Western Blot KITS FOR PARASITOLOGY and MYCOLOGY

Going native: Direct high throughput screening of secreted full-length IgG antibodies against cell membrane proteins

Gel microdroplet - fluorescence activated cell sorting (GMD-FACS) is an innovative high throughput screening platform for recombinant protein libraries, and we show here that GMD-FACS can overcome many of the limitations associated with conventional screening methods for antibody libraries. For example, phage and cell surface display benefit from exceptionally high throughput, but generally req...

ARE IgG-ANTI-FAB2ɤ AND IgG-ANTI-HINGE ANTIBODIES ACTIVE CONSTITUENTS OF INTRAVENOUS IMMUNOGLOBULIN?

Our aim was to find out if immunoglobulins for intravenous use (IVIG) contain IgG-anti-F(ab')2γ (AFab2) and IgG-antiHinge (AH) antibodies (Ab.) and to discuss how AFab2, in particular AH could contribute to immunomodulatory effect of IVIGs. We measured IgG, AFab2 and AH in five IVIG products approved for therapeutic use. IVIG showed higher AFab2 and AH concentration than sera of randomized heal...

The MHC class I-like IgG receptor controls perinatal IgG transport, IgG homeostasis, and fate of IgG-Fc-coupled drugs.

Abs of the IgG isotype are efficiently transported from mother to neonate and have an extended serum t(1/2) compared with Abs of other isotypes. Circumstantial evidence suggests that the MHC class I-related protein, the neonatal FcR (FcRn), is the FcR responsible for both in vivo functions. To understand the phenotypes imposed by FcRn, we produced and analyzed mice with a defective FcRn gene. T...